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Phone : +33 561285525

Key words

Experimental evolution, adaptive potential, fitness measure, genomic polymorphisms, transcriptomic variation, bacterial plant pathogen, Raltonia solanacearum, pathogen effectors

Firstname name

DR1 CNRS, Head of RAP team

RESEARCH SUBJECTS

Ralstonia solanacearum pathogenicity and adaptation to plants.

TRAINING

  • 1993  PhD from the Paris XI-Orsay University

  • 1993-1995 Post-doctoral Fellow at the Department of Genetics at the University of Munich investigating Ustilago maydis filamentous growth, under the direction of Prof. Regine Kahmann.

SCIENTIFIC PRODUCTION

 
 
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Phone

Tel +33(0)561285592

Key words

Firstname name

Function

RAP team

RESEARCH SUBJECTS

Research topics

TRAINING

Recent funding

RECENT FUNDING

Recent funding

COLLABORATIONS

Collaborations

COMMITTEE MEMBER

Committee member

SCIENTIFIC PRODUCTION

Scientific productions

CONNECTIONS

Science & Society

SCIENCE AND SOCIETY

Science & Society

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Firstname name

Researcher

RAP team

RESEARCH SUBJECTS

I am studying the molecular bases of adaptation to the host plant in the plant pathogen Ralstonia solanacearum using an experimental evolution approach. For that purpose, the GMI1000 strain was evolved by serial passage experiments on a fixed plant line during more than 300 generations. This experiment was conducted on 5 different plant species, and 5 evolved bacterial lineages per plant were generated. I am analyzing both genetic and epigenetic modifications involved in adaptive processes.

Phone

+33 (0)5 61 28 55 18

TRAINING

Key words

Bacterial adaptation, experimental evolution, adaptative mutation, genomic, transcriptomic, bacterial epigenetic, DNA methylation, plant pathogen bacteria, Ralstonia solanacearum

2019                  HDR (Habilitation à Diriger les Recherches) at Toulouse 3 University

2008-2010         Post-doctoral Fellow at LIPME, Toulouse, on experimental evolution                              of Ralstonia solanacearum

2004-2008         Post-doctoral Fellow at CIRAD La Réunion, on comparative genomic                            in Ralstonia solanacearum

2001-2004         Post-doctoral Fellow at Uppsala University, Sweden, on population                                dynamics in the fungi Daldinia loculata and Lactarius mammosus

1997-2000         Thesis in LEM, Lyon 1 University, on populations of the                                                    ectomycorrhizal fungus Hebeloma cylindrosporum

1997                  DEA Microbial Ecology at Lyon 1 University

RECENT FUNDING

2017-2023         Role of Epigenetic Modifications in a Plant Pathogen (ANR PRC)

2017                  Epigenetic modifications during host adaptation to resistant tomato                                    (FRAIB - LabEx TULIP)

2016-2017         Discrimination of bacterial population by their DNA‐methylation                                           pattern (INRA SPE)

2015-2016         Role of the transcription regulator, efpR, in Ralstonia solanacearum                                      (INRA SPE)

COLLABORATIONS

-Delphine Capela and Philippe Remigi, LIPME, Toulouse

-Yann Pécrix and Stéphane Poussier, UMR PVBMT, CIRAD, Saint-Pierre, La Réunion

-Julien Brillard and Alain Givaudan, DGIMI, Montpellier, France

-Pédro Oliveira, Genoscope, Evry, France

COMMITTEE MEMBER

2020-2024       Management of the Organizing Committee for the National Congress                             « Rencontres Plantes Bactéries », Aussois, 24 - 28 January 2022 / 28                                   January - 2 February 2024

2020-2024       Member of the Educational Council of TULIP LabEx, Toulouse

2015-2020       Member of the Scientific Council of TULIP LabEx, Toulouse

2016                Co-head of Organizing Committee for the International Congress

                        «International Bacterial Wilt Symposium », Toulouse, 3-7 juillet 2016.

SCIENTIFIC PRODUCTION

My publications via ORCID (0000-0001-5282-4157)

Gopalan-Nair R, Jardinaud M-F, Legrand L, Landry D, Barlet X, Lopez-Roques C, Vandecasteele C, Bouchez O, Genin S, Guidot A. (2020) Convergent Rewiring of the Virulence Regulatory Network Promotes Adaptation of Ralstonia solanacearum on Resistant Tomato. Molecular Biology and Evolution [Internet]. Available from: https://doi.org/10.1093/molbev/msaa320

Perrier A, Barlet X, Rengel D, Prior P, Poussier S, Genin S, Guidot A (2019) Spontaneous mutations in a regulatory gene induce phenotypic heterogeneity and adaptation of Ralstonia solanacearum to changing environments. Environmental Microbiology, 21:3140-3152.

Guidot A (2019) Comment un pathogène bactérien change d’hôte ? In : 101 Secrets du Vivant, CNRS Editions.

Perrier A, Barlet X, Peyraud R, Rengel D, Guidot A, Genin S (2018) Comparative transcriptomic studies identify specific expression patterns of virulence factors under the control of the master regulator PhcA in the Ralstonia solanacearum species complex. Microbial Pathogenesis, 116:273-278.

Morel A, Peeters N, Vailleau F, Barberis P, Jiang G, Berthomé R, Guidot A (2018) Plant pathogenicity phenotyping of Ralstonia solanacearum strains. Methods in Molecular Biology, 1734:223-239.

Erill I, Puigvert M, Legrand L, Guarischi-Sousa R, Vandecasteele C, Setubal JC, Genin S, Guidot A, Valls M (2017) Comparative analysis of Ralstonia solanacearum methylomes. Frontiers in Plant Science, 8:504. doi: 10.3389/fpls.2017.00504.

Jiang G, Peyraud R, Remigi P, Guidot A, Berthomé R, Ding W, Jousset A, Genin S, Peeters N (2016) The population dynamics of a bacterial pathogen after host re-infection affects the founding population size. bioRxiv, 061408; doi: https://doi.org/10.1101/061408.

Perrier A, Peyraud R, Rengel D, Barlet X, Lucasson E, Gouzy J, Peeters N, Genin  S, Guidot A (2016) Enhanced in planta fitness through adaptive mutations in EfpR, a dual regulator of virulence and metabolic functions in the plant pathogen Ralstonia solanacearum. PloS Pathogens, 12(12): e1006044.doi:10.1371/journal.ppat.1006044.

Reboud X, Sicar, D, Bataillon T, Bedhomme S, Dillmann C, Gaba S, Gallet R, Guidot A, Goldringer I, Jasmin JN, Kaltz O, Méry F, Nidelet T, Schneider D, Spor A (2016). Evolution expérimentale. In: Thomas F., Raymond M., Lefevre T., Biologie évolutive (p. 755-790). De Boeck Supérieur Sciences (DBS Sciences). 1000 p.

Pensec F, Lebeau A, Daunay MC, Chiroleu F, Guidot A, Wicker E (2015) Towards the identification of Type III effectors associated with Ralstonia solanacearum virulence on tomato and eggplant. Phytopathology, 105:1529-1544.

Guidot A, Jiang W, Ferdy JB, Thébaud C, Barberis P, Gouzy J,  Genin S (2014) Multihost experimental evolution of the pathogen Ralstonia solanacearum unveils genes involved in adaptation to plants. Molecular Biology & Evolution, 31:2913-2928.

Peeters N, Guidot A, Vailleau F, Valls M (2013) Ralstonia solanacearum, a widespread bacterial plant pathogen in the post-genomic era. Molecular Plant Pathology, 14, 651-662.

Remigi P, Anisimova M, Guidot A, Genin S, Peeters N (2011) Functional diversification of the GALA type III effector family contributes to Ralstonia solanacearum adaptation on different plant hosts. New Phytologist, 192, 976–987.

Coupat-Goutaland B, Bernillon D, Guidot A, Prior P, Nesme X, Bertolla F (2011) Ralstonia solanacearum virulence increased following large interstrain gene transfers by natural transformation. Molecular Plant Microbe Interaction, 24, 497-505.

Macho* AP, Guidot A*, Barberis P, Beuzón CR, Genin S (2010) A competitive index assay identifies several Ralstonia solanacearum Type III effector mutant strains with reduced fitness in host plants. Molecular Plant Microbe Interaction, 23, 1197-1205. *Both authors contributed equally to this work

Remenant B, Coupat-Goutaland B*, Guidot A*, Cellier G, Wicker E, Allen C, Fegan M, Pruvost O, Elbaz M, Calteau A, Salvignol G, Mornico D, Mangenot S, Barbe V, Medigue C, Prior P (2010) Genomes of three tomato pathogens within the Ralstonia solanacearum species complex reveal significant evolutionary divergence. : three original genome sequences for new insights of the species complex. BMC Genomics, 11, 379-395. *Both authors contributed equally to this work

Guidot A, Elbaz M, Carrère S, Siri MI, Pianzzola MJ, Prior P, Boucher C (2009) Specific genes from the potato brown rot strains of Ralstonia solanacearum and their potential use for strain detection. Phytopathology, 99, 1105-1112.

Guidot A, Coupat B, Fall S, Prior P, Bertolla F (2009) Horizontal gene transfer between Ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays. The ISME Journal, 3:549-562.

Högberg N, Guidot A, Jonsson M, Dahlberg A (2009) Microsatellite markers for the ectomycorrhizal basidiomycete Lactarius mammosus. Molecular Ecology Resources, 9:1008-1010.

Guidot A, Prior P, Schoenfeld J, Carrère S, Genin S, Boucher C (2007) Genomic structure and phylogeny of the plant pathogen Ralstonia solanacearum inferred from gene distribution analysis. Journal of Bacteriology, 189:377-387.

Guidot A, Verner MC, Debaud JC, Marmeisse R (2005) Intraspecific variation in use of different organic nitrogen sources by the ectomycorrhizal fungus Hebeloma cylindrosporum. Mycorrhiza, 15:67-177.

Marmeisse R, Guidot A, Gay G, Lambilliotte R, Sentenac H, Combier JP, Melayah D, Fraissinet-Tachet L, Debaud JC (2004) Hebeloma cylindrosporum - a model species to study ectomycorrhizal symbiosis from gene to ecosystem. New Phytologist, 163:481-498.

Guidot A, Johannesson H, Dahlberg A, Stenlid J (2003) Parental tracking in the postfire wood decay ascomycete Daldinia loculata using highly variable nuclear gene loci. Molecular Ecology, 12:1717-1730.

Guidot A, Debaud JC, Marmeisse R (2002) Spatial distribution of the below-ground mycelia of an ectomycorrhizal fungus inferred from specific quantification of its DNA in soil samples. FEMS Microbiology Ecology, 42:477-486.

Guidot A, Debaud JC, Effosse A, Marmeisse R (2004) Below-ground distribution and persistence of an ectomycorrhizal fungus. New Phytologist, 161:539-547.

Guidot A, Debaud JC, Marmeisse R (2003) Nouvelles approches pour l’étude des populations de champignons ectomycorhiziens: typage génétique des mycorhizes et analyse de l’ADN du sol. Les Actes du BRG, 4, 479-489.

Guidot A, Gryta H, Gourbière F, Debaud JC, Marmeisse R (2002) Forest habitat characteristics affect balance between sexual reproduction and clonal propagation of the ectomycorrhizal mushroom Hebeloma cylindrosporum. Oikos, 99:25-36.

Guidot A, Debaud JC, Marmeisse R (2001) Correspondence between genet diversity and spatial distribution of above- and below-ground populations of the ectomycorrhizal fungus Hebeloma cylindrosporum. Molecular Ecology, 10:1121-1131.

Guidot A, Lumini E, Debaud JC, Marmeisse R (1999) The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on Pinus root systems. Applied and Environmental Microbiology, 65:903-909.

 
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Phone : 335 61 28 55 25

Key words

Systems biology, mathematical modeling, metabolic modeling, metabolism, trophism, tomato plant, Ralstonia solanacearum, Xylella fastidiosa

Firstname name

Researcher INRAE

Team : RAP

RESEARCH SUBJECTS

I study the link between metabolism and virulence. To this end, I used systems biology and in particular metabolic modelling. I also started the modelling of the infection’s dynamics of plant pathogens. My work time is split between modelling and experimentations to calibrate my models.

TRAINING

2014 – 2016 : Postdoctorate at the Physiology and Biotechnology of Algae laboratory (IFREMER), including 6 months as a visiting scientist at Imperial College of London (dtp Chemical engineering)

2011 – 2014 : PhD INRAE - Inria at the Laboratory of Environmental Biotechnology at INRAE Narbonne and in the team BIOCORE of Inria Sophia-Antipolis.

2010 – 2011 : Bioinformatician, Mount Sinai School of Medicine (New York)

2009 - 2010 : MSc Computing for Biomedical Applications, Imperial College of London.

2007 - 2010 : Ecole Centrale de Lyon (general engineering degree).

2005 – 2007 : Training classes in mathematics, physics and computing (MPSI, MP*)

RECENT FUNDING

Recent funding

COLLABORATIONS

Collaborations

COMMITTEE MEMBER

Elected member of the scientific council of SPE dpt.

Elected member of the scientific council of TULIP LABEX.

Elected member of the laboratory council.

SCIENTIFIC PRODUCTION

My authored publications via ORCID.

HOBBIES

On a non-professional level, one of my favorite hobby is to hold a blog on cooking and pastry.

 
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Phone

Recent funding

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RECENT FUNDING

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RAP_LeoGerlin_WEB_edited.jpg

Phone

+33 5 61 28 50 45

Key words

Systems biology, metabolic modeling, plant pathogens, tomato, xylem, Xylella fastidiosa, Ralstonia solanacearum, metabolic networks

Léo GERLIN

Function

PhD student UPS / ED SEVAB (2018 - 2021)

RESEARCH SUBJECTS

Study of metabolic interactions between plant and xylem-colonizing bacteria using constraint-based modeling

TRAINING

INSA Toulouse – Biological Engineering (MSc)

EDUCATION

- Practical Courses - Solution Chemistry - INSA Toulouse 1ère année (2018 - 2020)

- Lecture and Practical Courses - Metabolic networks and modeling – Université Paul Sabatier  – MSc Bioinformatics and Systems Biology (2018 - 2020)

SCIENTIFIC PRODUCTION

 
 
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Firstname name

Function

Team

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TRAINING

Recent funding

Phone

Recent funding

Key words

RECENT FUNDING

COLLABORATIONS

Collaborations

EDUCATION

Teachings

COMMITTEE MEMBER

Committee member

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Scientific productions

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SCIENCE AND SOCIETY

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OTHER AFFILIATION

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