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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
Arabidopsis
transformation of Arabidopsis thaliana
METHODS
Transgenic plant production
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We perform routine transformations and regeneration of stable transgenic for Arabidopsis thaliana and Medicago truncatula, via Agrobacterium tumefaciens.
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for Medicago truncatula, the stable transgenic lines are obtained via a regeneration via embryogenesis (Chabaud et al. 1996)
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For Arabidopsis thaliana transformation we use the the "floral dip" method (Bechtold et al. 1993)
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Tomato is transformed with Argrobacterium tumefaciens starting with cotyledons.
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For Brachypodium distachyon, the model monocotyledon plant, the transformation technique was transferred from our colleagues at INRAE Versailles. It consist in the Agrobacterium tumefaciens mediated transformation via somatic embryogenesis.
In developement
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In collaboration with a team at LIPME, the service developed a quick method for the transient expression of Medicago truncatula roots (Rembliere et al. 2018).
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The service has developed Medicago truncatula mutant lines that are more embryogenic in order to increase the transformation efficiency.
trainees
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